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. Author manuscript; available in PMC: 2007 Nov 13.
Published in final edited form as: Pain. 2007 Feb 2;131(1-2):83–95. doi: 10.1016/j.pain.2006.12.018

Figure 1. Microinjection sites within the RVM.

Figure 1

Photomicrographs showing placement of A) midline single cannula tract (bregma – 10.04 mm) and B) bilateral cannula tracts (bregma – 10.80 mm) within the RVM in brain sections counterstained with cresyl violet. Section thickness = 40 μm. Brightfield photomicrographs of Y1 receptor immunoreactivity in the RVM at C) bregma – 10.08 mm and D) bregma – 10.80 mm. Scale bars = 200 μm for C, D. The boxed areas in C and D represent areas of higher magnification as shown in E and F, respectively. Scale bars = 100 μm for E, F.