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. Author manuscript; available in PMC: 2007 Nov 13.
Published in final edited form as: Virology. 2007 May 11;366(1):84–97. doi: 10.1016/j.virol.2007.02.041

Fig. 1.

Fig. 1

Structure of MVA/KB9-5, expression of SHIV genes, and study protocol. (A) Env and tat from SHIV/KB9 (gray boxes) and gagpol and nef from SIVmac239 (white boxes) were inserted into deletions II and III (black boxes) of MVA to form MVA/KB9-5. Chimeric Tatnef protein was expressed under control of the modified P7.5 promoter; Env and Gagpol were expressed under control of the modified H5 promoter. (B) SHIV antigens were immunoprecipitated from cells infected with MVA/KB9-5 with: monoclonal antibody T8 (α-env), SIV polyclonal serum E544 (α-SIV), and HIV-1BH10 Tat antiserum (α-tat). Precursor and cleavage products for Env and Gag are shown. The Tatnef protein was precipitated by both Tat and Nef (E544) antibodies. (C) Study timeline.