Table 1.

Densitometric analysis of CEBPA isoforms detected by Western blotting

	p42/p30 ratio			Total CEBPA (p42+p30)			p42
	DMSO	CDDO	DMSO/CDDO	DMSO	CDDO	CDDO/DMSO	DMSO	CDDO	CDDO/DMSO
Figure 3D	0.54	1.53	2.85	1.89	1.77	0.93	0.66	1.07	1.62
Figure 4A	0.98	2.01	2.05	2.74	2.20	0.80	1.36	1.47	1.08
Figure 4B	0.83	1.95	2.36	1.45	1.87	1.29	0.66	1.24	1.89
Figure 5A	1.04	3.41	3.26	1.71	1.45	0.84	0.87	1.12	1.28
Figure 5F	0.47	1.36	2.91	1.13	1.59	1.42	0.36	0.92	2.56
Figure 6A	0.06	1.45	23.49	0.65	1.20	1.86	0.04	0.71	18.92
Figure 6C	1.33	2.34	1.76	1.54	1.11	0.72	0.88	0.78	0.89
Figure 6D	0.43	0.96	2.22	1.45	2.03	1.41	0.44	1.00	2.28
Mean	0.71	1.88	5.11	1.57	1.65	1.16	0.66	1.04	3.81
SD	0.41	0.75	7.44	0.61	0.39	0.40	0.40	0.25	6.13
t test	—	0.002	—	—	0.744	—	—	0.037	—

This table shows the densitometric analysis of p42 and p30 CEBPA isoform and loading control bands as detected by Western blotting in Figures 3 through 6. DMSO and CDDO designate the type of stimulation of the HL60 cells, and the ratio of CDDO-induced changes is depicted as the DMSO/CDDO fraction. The p42/p30 ratio was calculated by dividing the densitometric values of p42 and p30. Total CEBPA (p42+p30) was calculated by addition of p42 and p30 bands and dividing this value by the densitometric value of the loading control band. p42 CEBPA was calculated by dividing the densitometric values of p42 and of the loading control band. Mean and standard deviation (SD) of all blots in the presented figures are shown, and DMSO- and CDDO-stimulated data were compared using a Student t test.

— indicates not applicable