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. 2007 Aug 15;110(10):3773–3779. doi: 10.1182/blood-2007-06-094565

Figure 1.

Figure 1

Gαi2 and PLC in neutrophils are required for neutrophil chemokine-induced arrest on P-selectin/ICAM-1. (A) Carotid cannulas were placed in Gnai2−/− mice (□) and Gnai2+/+ mice (■) and connected to autoperfused flow chambers coated with P-selectin and ICAM-1 alone or in combination with CXCL1 (10 μg/mL). The wall shear stress in all flow chamber experiments was 5.94 dyn/cm2. There were at least 3 mice and 4 flow chambers per group. To account for the 2.5-fold increase of neutrophils in Gnai2-deficient mice compared with littermate controls (Table 1, the number of adherent neutrophils was normalized to systemic neutrophil counts. Number of normalized adherent cells per field of view is presented as means plus or minus SEM. (B) Number of adherent neutrophils on P-selectin and ICAM-1 in combination with CXCL1 of U73122-pretreated whole blood after 6 minutes. Data presented as means plus or minus SEM from 3 mice. #P < .05.