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. Author manuscript; available in PMC: 2008 Sep 1.
Published in final edited form as: J Cyst Fibros. 2007 Feb 27;6(5):351–359. doi: 10.1016/j.jcf.2007.01.001

Figure 4. Quinine blocks CFTR dependent Cl− secretion at concentrations that do not alter airway epithelial resistance.

Figure 4

A: In the presence of a Cl− gradient, apical quinine added at 100 μg/mL led to a modest change (7.4 μA/cm2) in ISC. After activation of CFTR with forskolin (20 μM), substantial reduction in short-circuit current was seen with apical quinine (100 μg/mL, *p<0.05, n=12, ±SEM). Addition of glybenclamide (Glyb, 200 μM) further reduced ISC(**p<0.005, n=12, ±SEM). In a reversed Cl− gradient, low dose quinine by itself had minimal effect (−5.8 μA/cm2, n=13, ±SEM), indicating functionally intact junctional complexes. B: No significant change in monolayer resistance was observed with 100 μg/mL quinine (n=12, ±SEM).