Table 3.
List of the top 15 most significantly expressed genes in MCF-7 cells or in skeletal muscle as determined using the Actichip microarray
| Gene name | Gene Accession number | Score (d) | Log2 expression ratio | |
| Genes mainly expressed in skeletal muscle | Nebulette (Actin-binding Z-disk protein) | NM_006393 | 42.93 | 3.81 |
| Troponin I, fast skeletal muscle (Troponin I, fast-twitch isoform) | NM_003282 | 34.39 | 2.60 | |
| Huntingtin interacting protein 1 (HIP-I) | NM_005338 | 33.55 | 2.59 | |
| Myosin light chain 2a | NM_021223 | 32.80 | 3.26 | |
| Troponin C, skeletal muscle | NM_003279 | 32.21 | 2.45 | |
| Myosin light chain 2 | NM_013292 | 29.25 | 3.06 | |
| Myosin heavy chain, skeletal muscle, perinatal | NM_002472 | 28.14 | 2.80 | |
| Troponin T, fast skeletal muscle isoforms | NM_006757 | 26.04 | 2.71 | |
| Myosin heavy chain, cardiac muscle beta isoform | NM_000257 | 24.94 | 3.12 | |
| Actin, alpha skeletal muscle (Alpha-actin 1) | NM_001100 | 24.21 | 3.50 | |
| Troponin C, slow | NM_003280 | 21.31 | 2.54 | |
| Nebulin | NM_004543 | 20.17 | 2.78 | |
| Smoothelin | NM_134269 | 19.50 | 1.32 | |
| Tropomyosin 1 alpha chain (Alpha-tropomyosin) | NM_000366 | 18.92 | 1.99 | |
| Myotilin | NM_006790 | 18.07 | 1.56 | |
| Genes mainly expressed in MCF-7 cells | Actin, cytoplasmic 2 (Gamma-actin) | NM_001614 | -30.24 | -2.58 |
| Elongation factor 1-beta (EF-1-beta) | NM_001959 | -22.14 | -1.26 | |
| Actin binding protein anillin, | NM_018685 | -20.12 | -1.13 | |
| Keratin, type I cytoskeletal 18 | NM_000224 | -18.78 | -2.83 | |
| Ankyrin 3 (ANK-3) (Ankyrin G) | NM_020987 | -16.43 | -2.17 | |
| Gamma-parvin | NM_022141 | -16.30 | -1.03 | |
| Spectrin beta chain, brain 2 (Spectrin, non-erythroid beta chain 2) (Beta-III spectrin) | NM_006946 | -16.28 | -0.84 | |
| Coronin 1B (Coronin 2) | NM_020441 | -15.97 | -0.84 | |
| Beta-centractin (Actin-related protein 1B) (ARP1B) | NM_005735 | -15.79 | -1.40 | |
| NB thymosin beta | NM_021992 | -14.92 | -1.42 | |
| Coronin 2A (WD-repeat protein 2) (IR10) | NM_052820 | -14.39 | -1.24 | |
| ARP2/3 complex 16 kDa subunit (P16-ARC) (Actin-related protein 2/3 complex subunit 5) | NM_005717 | -13.74 | -1.06 | |
| Tight junction protein ZO-1 (Zonula occludens 1 protein) (Zona occludens 1 protein) (Tight junction protein 1) | NM_003257 | -13.70 | -1.28 | |
| Transcription factor 7 (T-cell-specific transcription factor 1) (TCF- 1) (T-cell factor 1) | NM_003202 | -13.60 | -0.67 | |
| Catenin delta-1 (p120 catenin) (p120(ctn)) (Cadherin-associated Src substrate) (CAS) (p120(cas)) | NM_001331 | -13.60 | -0.77 |
The gene list was established from our dataset through SAM analysis [64]. In SAM, the significant genes are represented on a plot by points displaced by a distance denoted as the score (d) from a line on which genes identified simply by chance are aligned. Log2 expression ratios were determined by averaging the Log2 ratios calculated from fluorescence intensities of 10 repeated hybridisations.