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Chromatographic and immunoprecipitation analyses confirm that ZAG does not bind β2m. (A) β2m (Top), ZAG (Middle), and a mixture of ZAG incubated with β2m (Bottom) were analyzed by size exclusion chromatography. (Inset) SDS/13% PAGE analysis of the peaks in the bottom panel. (B) Aliquots of ZAG, serum, and β2m were immunoprecipitated with either anti-ZAG or anti-β2m antibodies. Precipitated proteins were analyzed by Western blot using antisera against either ZAG or β2m.
