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Specific cytotoxicity of Fab 13.4.1–PE38 to APCs. Competition of Fab 13.4.1–PE38 cytotoxicity by recombinant purified Fab 13.4.1. RMA-S⋅Kk cells were incubated overnight with 0.1 mM Ha and NP peptides at 26°C. Cells were then incubated for 20 hr with recombinant Fab 13.4.1–PE38. In competition experiments, peptide-loaded RMA-S⋅Kk cells were preincubated for 15 min with 50 μg/ml recombinant purified Fab 13.4.1 or control antibody HB21.
