Table 1.

TG content of pancreas, skeletal muscle, and liver of hyperleptinemic rats and pairfed and free-feeding controls

Tissue	AdCMV–β-gal Wistar	AdCMV–leptin Wistar	Pairfed Wistar*
Pancreas	8.86  ±  1.86  (3)	0.47  ±  0.39  (6)	3.63  ±  2.00  (3)
Skeletal muscle	4.12  ±  1.16  (4)	0.33  ±  0.12  (4)	1.31  ±  0.26  (5)
Liver	4.82  ±  1.03  (5)	0.64  ±  0.19  (4)	4.59  ±  1.17  (5)

Data are given as mean ± SEM. AdCMV–leptin infusion resulted in a rise in plasma leptin level from 1.3 ± 0.3 ng/ml before to 12 ± 1.9 ng/ml 3 days after the infusion. Leptin levels were 14.7 ± 2.2 and 13.7 ± 2.1 ng/ml at 7 and 14 days postinfusion, respectively. In the control groups, plasma levels were invariably below 1.5 ng/ml. Total lipids were extracted from ≈100 mg of tissue as described (22) and frozen under liquid nitrogen. TG was extracted with 30 μl of tert-butyl alcohol and 20 μl of a Triton X-104/methyl alcohol mixture (1:1, vol/vol). TG content was measured by a Sigma Triglyceride (GPO-Trinder) kit as described (5). Number of experiments is in parentheses. TG content in milligrams per gram of wet weight of tissue. 

^*Pairfed AdCMV–β-gal-infused Wistar rats did not appear to differ in terms of body fat from intact pairfed rats.