Figure 1 Immunoblot of a recombinant amino (NH2) terminal region of α enolase (NAE) with sera from an ataxic patient with Hashimoto's encephalopathy. M, molecular weight marker; lane 1, recombinant NAE protein; lane 2, serum from a positive control (patient with Hashimoto's encephalopathy, lanes 3, serum from the present case before steroid treatment; lane 4, serum from the present case after steroid treatment; lane 5, serum from a normal control. Arrowheads indicate the position of NAE. Sera were diluted 300‐fold. A strong signal against the NAE was detected in serum from the present case, and became weaker after steroid administration.