Figure 1.

Distribution of serum lipoprotein cholesterol in apoE(−/−)→apoE(+/+) experimental mice and apoE(+/+)→apoE(+/+) control mice after 13 weeks on the atherogenic diet. Gel filtration chromatography was performed using a Superose 6 column (Pharmacia) on a Waters 600 FPLC system. A 100-μl aliquot of mouse serum was injected onto the column and separated as described above. Cholesterol determinations were done with the Sigma kit 352 in the microplate assay. Data points represent mean values for cholesterol from five control and three experimental mice.