Figure 2.

Mean area of the aortic atherosclerotic lesions per section per animal for the apoE(−/−) →apoE(+/+) experimental mice and apoE(+/+)→apoE(+/+) control mice on the atherogenic diet. The analysis was performed on alternate 10 μm sections starting at the end of the aortic sinus and extending 300 μm distally. Frozen sections (10 μm) were fixed with 4% paraformaldehyde and stained with oil red O. The areas of lipid-stainable lesions were measured by digitizing morphometry in a blinded fashion. Data are from 14 experimental apoE(−/−)→apoE(+/+) mice and 11 control apoE(+/+)→ apoE(+/+) mice after 13 weeks on the atherogenic diet.