Table 1.
Total serum lipid levels in female C57BL/6J mice after transplantation with apoE(−/−) or apoE(+/+) bone marrow
| Donor mouse | n | Serum lipid level, mg/dl
|
|||||
|---|---|---|---|---|---|---|---|
| Baseline post-BMT
|
Atherogenic diet
|
||||||
| 6 weeks
|
13 weeks
|
||||||
| Chol | Trigl | Chol | Trigl | Chol | Trigl | ||
| apoE(+/+) | 14 | 70 ± 23 | 64 ± 35 | 194 ± 25 | 66 ± 32 | 220 ± 34 | 41 ± 9 |
| apoE(−/−) | 11 | 77 ± 14 | 61 ± 30 | 195 ± 36 | 70 ± 60 | 229 ± 32 | 44 ± 17 |
The baseline values were obtained on a diet of 4.5% fat rodent chow (Rodent Diet 5001, PMI) 8 weeks post-BMT. The atherogenic high fat diet consisted of 19.5% fat, 1.25% cholesterol, and 0.5% cholic acid (Butter Fat Diet, ICN). At the indicated time points, blood was obtained from nonfasting mice. Serum cholesterol (Chol) and triglyceride (Trigl) determinations were done with Sigma kits 352 and 339, adapted for microplate assay. Absorbance was read on a Molecular Devices microplate reader. Values are mean ± SEM. The number of mice in each group is indicated by n. Differences between groups were not statistically significant at any time point.