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(A) ADP-ASAP. (B) Phosphorimage of the radioactive cleavage assays. Cleavage reactions were carried out with 5 nM ADP-ASAP, 1 µM MgZ and 1 mM ADP (in the case of ADP-induced cleavage) in 50 mM HEPES (pH 7.0), 20 mM MgCl2, 0.001% Tween-20 at room temperature. ▸, substrate; ▹, 3′ cleavage fragment. (C) Fluorescence kinetics in response to various ADP concentrations. Similar reaction conditions in panel B were applied.
