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. Author manuscript; available in PMC: 2008 Oct 15.
Published in final edited form as: Bioorg Med Chem Lett. 2007 Aug 16;17(20):5548–5551. doi: 10.1016/j.bmcl.2007.08.024

Table 1.

Comparison of CaaX Substrate Predictions

CaaX Protein Rel. fluor. increase1 Beese prediction3 PREN base4 PREN base XCVLS5
CRIS Protein phosphatase 1 reg (in hi bi tor) subunit 16 B 886 H +
CKIS Rab 40 A 574 H* ++
CQTS DNA J 508 K +
CVLS H- Ras 388 K ++
CVIS Transducin gamma subunit 331 K ++
CTIS Guanylate binding protein 1 interferon - inducible 325 K ++
CFPS CCNG 2 : cyclin G2 280 H − − −
CLIS Phosphorylase kinase B 218 K ++
CLVS G protein coupled receptor kinase 1 217 K ++
CSVS Inositol polyphosphate - 5- phosphatase 180 H +
CRPS WDTC 1 171 H +
CTFS Xylosyl protein beta 1 ,4 -galactosyltransferase 166 H − − +
CRQS Unknown protein fragment (Q 15693) 121 NH − ++
CVHS Galectin - 12 100 NH − − +
CKSS Unknown Fragment ( q 12814 ) 82 NH − − −
CFSS Collagen type V alpha 3 subunit 60 NH − ++
CDMS Topo I binding Arg/Ser rich 42 H* − − +
CAKS Rab 38 332 NH − − ++
CEGS Protein with 8 zinc finger domains 28 NH − − ++
CQKS cytidine and deoxycytidylate deaminase 262 NH − ++
CVES Unknowm fragment (Q 29856) 20 NH − − − −
CPAS Ribosomal protein L 12 19 NH − − +
CGAS Kinesin family member 22 6 NH − − +
CAES G protein –coupled receptor 41 62 NH − − ++
CIRS Rab 3 interacting protein 1 0 NH − ++
CLRS Globoside alpha – 13 – N - acetylgalactosam inyltra nsferase1 0 NH − − +
CFNS Unknown Fragment (Q 14922) 0 NH − − −
1

Relative fluorescence increase was determined using a PerkinElmer Fusion plate reader (Ex 335/Em 535; 30 minute values reported) and scaled to the fluorescence increase observed for CVLS.

2

In these cases, the fluorimetric indication of farnesylated peptide product was not conclusively confirmed by HPLC analysis.

3

Predictions derived form the FTase substrate model reported by Reid et al. K: known substrate; H: hypothesized substrate; H*: not reported as a substrate by Reid et al., but consistent with their rules for substrate ability; NH: not hypothesized to be a substrate.

4

PRENbase prediction for the sequence XXXXXXXXCaaS, as described in the text.

5

PRENbase prediction for the sequence XXXXXXXXCVLS, as described in the text.