TABLE 2.
Functional Gene Ontology annotations of the S. mansoni egg secretome. GO annotations for the most abundant egg secreted proteins (22 proteins, Top 50% ESP) are compared to the GO annotations for both the entire egg secretome identified in this study (188 proteins, Total ESP) and the approximately 650 non-fragmentary schistosome proteins of the NCBI non-redundant data bank (NCBI nr).
| Top 50% ESP | Total ESP | NCBI nr* | ||||
|---|---|---|---|---|---|---|
| GO categories and subcategories | Total (22) | % total | Total (188) | % total | Total (650) | % Total |
| GO:0003674 molecular function | 19 | 100% | 129 | 100% | 448 | 100% |
| GO:0003774 motor activity | 0 | 0% | 3 | 2% | 29 | 6% |
| GO:0003824 catalytic activity | 14 | 74% | 79 | 61% | 267 | 60% |
| GO:0016491 oxidoreductase activity | 5 | 26% | 24 | 19% | 91 | 20% |
| GO:0016740 transferase activity | 3 | 16% | 18 | 14% | 73 | 16% |
| GO:0016787 hydrolase activity | 4 | 21% | 29 | 22% | 90 | 20% |
| GO:0004871 signal transducer activity | 2 | 11% | 23 | 18% | 20 | 4% |
| GO:0005198 structural molecule activity | 3 | 16% | 8 | 6% | 20 | 4% |
| GO:0005215 transporter activity | 3 | 16% | 19 | 15% | 83 | 19% |
| GO:0005488 binding | 13 | 68% | 102 | 79% | 278 | 62% |
| GO:0000166 nucleotide binding | 5 | 26% | 32 | 25% | 77 | 17% |
| GO:0005496 steroid binding | 1 | 5% | 2 | 2% | - | - |
| GO:0005515 protein binding | 10 | 53% | 70 | 54% | 49 | 11% |
| GO:0043167 ion binding | 3 | 16% | 26 | 20% | 91 | 20% |
| GO:0016209 antioxidant activity | 3 | 16% | 6 | 5% | 26 | 6% |
| GO:0004601 peroxidase activity | 1 | 5% | 4 | 3% | 8 | 2% |
| GO:0004791 thioredoxin-disulfide reductase act. | 2 | 11% | 3 | 2% | - | - |
| GO:0030234 enzyme regulator activity | 2 | 11% | 13 | 10% | 10 | 2% |
| GO:0008150 biological_process | 17 | 100% | 122 | 100% | 422 | 100% |
| GO:0000003 reproduction | 1 | 6% | 3 | 2% | 126 | 30% |
| GO:0007275 development | 2 | 12% | 14 | 11% | 76 | 18% |
| GO:0007582 physiological process | 16 | 94% | 117 | 96% | 330 | 78% |
| GO:0008152 metabolism | 16 | 94% | 101 | 83% | 217 | 51% |
| GO:0016265 death | 6 | 35% | 22 | 18% | - | - |
| GO:0051179 localization | 4 | 24% | 37 | 30% | - | - |
| GO:0007154 cell communication | 6 | 35% | 30 | 25% | 73 | 17% |
| GO:0050789 regulation of biological process | 6 | 35% | 45 | 37% | 6 | 1% |
| GO:0050896 response to stimulus | 4 | 24% | 26 | 21% | 34 | 8% |
| GO:0006950 response to stress | 3 | 18% | 19 | 16% | - | - |
| GO:0005575 cellular_component | 15 | 100% | 108 | 100% | 335 | 100% |
| GO:0005576 extracellular region | 3 | 20% | 14 | 13% | 13 | 4% |
| GO:0005623 cell | 14 | 93% | 102 | 94% | 279 | 83% |
| GO:0043226 organelle | 7 | 47% | 56 | 52% | - | - |
| GO:0043234 protein complex | 4 | 27% | 31 | 29% | - | - |
| GO:0000015 phosphopyruvate hydratase complex | 1 | 7% | 8 | 7% | - | - |
| GO:0005667 transcription factor complex | 3 | 20% | 3 | 3% | - | - |
*
as shown in Knudsen et al. 200639