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Determination of heparanase levels in blood. Blood samples were obtained from control healthy volunteers and allowed to clot to generate serum (n = 20) or collected into tubes containing sodium citrate (n = 20) or EDTA (n = 18) as anticoagulants to produce plasma. Samples were diluted 1:2 with PBS and heparanase levels were quantified by a sandwich ELISA method, as described in the Materials and Methods section.
