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. Author manuscript; available in PMC: 2008 Oct 25.
Published in final edited form as: Virology. 2007 Jul 13;367(2):324–333. doi: 10.1016/j.virol.2007.06.011

Fig. 7. Iron chelators inhibit phosphorylation of RNAPII.

Fig. 7

(a) Chelators do not have an effect on expression of CDK9. 293 cells were treated for 24 h with 10 μM 311 or 100 μM of ICL670. The cells were lysed were resolved on 10% SDS-PAGE, and immunoblotted anti-CDK9 antibodies. Lane 1: control untreated cells; lanes 2, 3 and 4: cells treated with DFO, 311 or ICL670. (b) Chelators inhibit association of CDK9 with cyclin T1. The cell lysates prepared as in (a) were subjected to immunoprecipitation with anti-cyclin T1 antibody (lanes 3–6). The immunoprecipitated material was resolved on 10% SDS-PAGE, and immunoblotted anti-CDK9 antibodies. Lane 1: input control untreated cells; lane 2: precipitation of untreated cells with non-specific preimmune IgG, lanes 3–6: immunoprecipitation with anti-cyclin T1 antibodies of lysates from untreated, DFO, 311 and ICL670 treated cells. The precipitated samples were resolved on 10% SDS-PAGE and immunoblotted with antibodies against CDK9. (c) Chelators inhibit phosphorylation of RNAPII. The cell lysates prepared as in (a) were resolved on 7.5% SDS-PAGE, and immunoblotted with the RNAPII CTD phospho-Serine 2 specific antibodies. Lane 1: control untreated cells; lanes 2,3 and 4: cells treated with DFO, 311 or ICL670.