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Characterization of NSE–CD46 transgenic mice. The NSE–CD46 construct consists of 2.8 kb of 5′ NSE regulatory sequence, NSE exon 1 and intron 1, and 15 bp of NSE exon 2 adjacent to a unique HindIII site. Polyadenylylation signals are provided by the simian virus 40 sequence at the 3′ end of the construct, which also facilitates identification of transgenic mice by slot-blot analysis of genomic DNA.
