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Amplification of CD46 message in transgenic brains. Total RNA was isolated from four line 52 transgenic (+) and three nontransgenic (−) littermate brain homogenates, reverse transcribed and PCR amplified (5) using 20-mer oligonucleotides within the CD46 sequence. Samples were incubated with and without RT to verify amplification of a cDNA–RNA substrate. Representative results from one transgenic and one nontransgenic brain are shown.
