Figure 3.

Cell surface expression of CD46 on dissociated, transgenic neurons. Hippocampi from NSE–CD46 transgenic mouse lines 21, 18, and 52 were removed, dissociated, and incubated with the anti-CD46 monoclonal antibody E4.3 (19). The solid areas in each panel represent neurons from nontransgenic littermate controls stained using the identical procedure. Each panel depicts fluorescence intensity (x axis) vs. cell number (y axis). These results were subsequently confirmed with two additional hippocampal preparations.