Figure 5.

Spread of MV in primary neurons and in vivo. (A) CD46+ primary neurons, infected for 48 hr with MV–Edmonston. (B) CD46+ primary neurons 96 hr after infection. Coverslips were fixed with 1:1 acetone:methanol and immunostained with a human SSPE serum as described. (C–E) NSE–CD46 neonatal (<24 postnatal) transgenic and nontransgenic mice were inoculated intracranially with 10⁵ pfu of MV–Edmonston and brain sections were immunostained for MV as described. Immunohistochemical staining for MV antigens in the cortical region (C) and in the hippocampus (D) of a representative, NSE–CD46 transgenic mouse infected with MV 10 days previously (×100). (E) Hippocampus of a nontransgenic mouse, inoculated 10 days previously (×100).