Figure 3.
Temperature and media effects on virus infection in isolated rabbit ventricular myocytes. (A) Percentage of cells positive for β-gal after 1 min to 8 hr exposure to 1.0 × 105 pfu of Adβgal in 1.0 ml Krebs solution at three different temperatures (8.9 × 104 cells per culture dish). Solid lines are logistical regression curves drawn through data. (B) Relative number of cells positive for β-gal, compared with infection in M199. Myocytes were exposed to 1.0 × 105 pfu of Adβgal in different media for 2.0 hr at 37°C (4.4 × 104 cells per culture dish). Whole rabbit blood had a hematocrit of 33%, and isolated red blood cells (RBC) were diluted in Krebs solution to a hematocrit of 33%. Iohexol was diluted in M199 to 20% by volume. (n = 3.)