Groups of mice were immunized with 1011 gc rAAV2/7gag and then treated with 200 μg anti-PD-1 antibody, twice prior to boost and 3 times after boost, 3 days apart, as described previously (36, 50, 51). Control mice were treated with 200 μg IgG2a. Mice were then immunized with 1010 vp AdC68gag, and 2 and 5 weeks later, lymphocytes were harvested from blood. Shown are density plots of live cells stained with an antibody to CD8 (x axis) and the tet (y axis). Numbers within plots denote tet+CD8+ cells as a percentage of CD8+ cells.