(A) To test for vector persistence after immunization, groups of mice were administered 1011 gc rAAV2/7gag or 1010 vp AdC68gag in the lower leg muscle. DNA was isolated on days 1, 7, 30, 240, and 270 and analyzed by gag nested real-time PCR. Copy numbers normalized to GAPDH for individual mice are shown; “X” denotes the group mean. (B) Cells from rAAV2/7gag- or AdHu5gag-immunized mice were transferred into RAG–/– mice immunized on day 1 or 7 with AdC68gag. Tet analyses were performed on PBMCs before and after AdC68gag immunization. Error bars represent SD for individual mice.