Figure 8. Coimmunization with adjuvant does not restore proliferative capability.

Groups of BALB/c mice were immunized with 10¹¹ gc rAAV2/7gag mixed in a 1:1 ratio with incomplete Freund’s adjuvant. Mice were then boosted with 10¹⁰ vp AdC68gag 1 month later, and blood was collected 2 and 4 weeks after the boost. Shown are density plots of live cells stained with an antibody to CD8 (x axis) and the gag-specific tet (y axis). Numbers within plots denote tet⁺CD8⁺ cells as a percentage of CD8⁺ cells.