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. 2007 Oct;64(10):673–680. doi: 10.1136/oem.2006.032078

Table 1 Oligonucleotide primers, probes and restriction endonuclease employed in genotyping (unless otherwise listed in the text).

Genes Molecular biology methods
PCR‐RFLP
Oligonucleotide primers Restriction endonuclease
DAT1 D9RU 5′‐CATCATCTACCCGGAAGCCA‐3′ DdeI
D9RL 5′‐CAGGGTGAGCAGCATGATGA‐3′
Real time PCR
Oligonucleotide primers Hybridisation probes
GSTP1 exon 6 Forward primer 5′‐AAGCAGAGGAGAATCTGGGACTC‐3′ 5′‐LC Red 640*‐ CCAGACACCACCATGTATCATCCTACTCTCp‐3′
(Reverse primer) 5′‐GGCCAGATGCTCACCTGGTC‐3′ 5′‐GTCATCCTTGCCCACCTCCT‐FL†3′
Pyrosequencing
Pyrosequencing primers Sequencing primers
MAO‐B 5′‐GTCCTTTAGGGAGCAGATTAG‐3′ 5′‐GATTAGAAGAAAGATGGT‐3′
5′‐biotin‐CAGACTCTGGTTCTGACTGC‐3′
SOD2 5′‐biotin‐CAGCCCAGCCTGCGTAGACG‐3′ 5′‐CCCAGATACCCCAAA‐3′
5′‐CTGGAGCCCAGATACCCCAA‐3′
PCR and dideoxy sequencing primers
EPHX3 5′‐GGAAACTGCCTTGCCACTCC‐3′
5′‐CTAGCTCTAAAGATGGACAGA‐3′
DHPLC (WAVE)
MAO‐A exon 8 5′‐TTGCTTGTGTGTGTTTTAGTGC‐3′
5′‐GACAGACCAAGATTTCTAATCC‐3′
NAT2 5′‐GGATTCATGCAGTAGAAATAC‐3′
5′ATAACGTGAGGGTAGAGAGG‐3′

*LightCycler Red 640; †fluorescein.