Table 1 Oligonucleotide primers, probes and restriction endonuclease employed in genotyping (unless otherwise listed in the text).
Genes | Molecular biology methods | |||
---|---|---|---|---|
PCR‐RFLP | ||||
Oligonucleotide primers | Restriction endonuclease | |||
DAT1 | D9RU 5′‐CATCATCTACCCGGAAGCCA‐3′ | DdeI | ||
D9RL 5′‐CAGGGTGAGCAGCATGATGA‐3′ | ||||
Real time PCR | ||||
Oligonucleotide primers | Hybridisation probes | |||
GSTP1 exon 6 | Forward primer 5′‐AAGCAGAGGAGAATCTGGGACTC‐3′ | 5′‐LC Red 640*‐ CCAGACACCACCATGTATCATCCTACTCTCp‐3′ | ||
(Reverse primer) 5′‐GGCCAGATGCTCACCTGGTC‐3′ | 5′‐GTCATCCTTGCCCACCTCCT‐FL†3′ | |||
Pyrosequencing | ||||
Pyrosequencing primers | Sequencing primers | |||
MAO‐B | 5′‐GTCCTTTAGGGAGCAGATTAG‐3′ | 5′‐GATTAGAAGAAAGATGGT‐3′ | ||
5′‐biotin‐CAGACTCTGGTTCTGACTGC‐3′ | ||||
SOD2 | 5′‐biotin‐CAGCCCAGCCTGCGTAGACG‐3′ | 5′‐CCCAGATACCCCAAA‐3′ | ||
5′‐CTGGAGCCCAGATACCCCAA‐3′ | ||||
PCR and dideoxy sequencing primers | ||||
EPHX3 | 5′‐GGAAACTGCCTTGCCACTCC‐3′ | |||
5′‐CTAGCTCTAAAGATGGACAGA‐3′ | ||||
DHPLC (WAVE) | ||||
MAO‐A exon 8 | 5′‐TTGCTTGTGTGTGTTTTAGTGC‐3′ | |||
5′‐GACAGACCAAGATTTCTAATCC‐3′ | ||||
NAT2 | 5′‐GGATTCATGCAGTAGAAATAC‐3′ | |||
5′ATAACGTGAGGGTAGAGAGG‐3′ |
*LightCycler Red 640; †fluorescein.