Table 2.
PCR and sequencing confirmation of CDSs identified as absent/divergent by CGH analysis
| PCR and sequencing | ||||||
| Gene ID | Gene name | Gene product | Intensity of Hybridization lower than with Twist | Genes deleted | Genes with nt variation(s) | Genes conserved |
| TWT018 | deoD | purine nucleoside phosphorylase | 1 | 1 | ||
| TWT041 | - | hypothetical protein | 7 | 7 | ||
| TWT099 | - | hypothetical protein | 3 | 3 | ||
| TWT101 | - | hypothetical protein | 1 | 1 | ||
| TWT151 | - | hypothetical protein | 1 | 1 | ||
| TWT158 | - | hypothetical protein | 3 | 3 | ||
| TWT171 | - | hypothetical protein | 2 | 2 | ||
| TWT176 | - | hypothetical protein | 1 | 1 | ||
| TWT199 | ftsE | cell division ATP-binding protein | 5 | 5 | ||
| TWT203 | ksgA | dimethyladenosine transferase | 1 | 1 | ||
| TWT232 | - | WiSP family protein | 3 | 3 | ||
| TWT277 | - | hypothetical protein | 1 | 1 | ||
| TWT311 | - | hypothetical protein | 1 | 1 | ||
| TWT386 | trpE | anthranilate synthase component I | 1 | 1 | ||
| TWT388 | - | hypothetical protein | 2 | 2 | ||
| TWT594 | - | WiSP family protein | 9 | 9 | ||
| TWT596 | - | WiSP family protein | 7 | 6 | 1 | |
| TWT604 | - | hypothetical protein | 2 | 1 | 1 | |
| TWT613 | - | hypothetical protein | 1 | 1 | ||
| TWT614 | ftsK | cell division protein FtsK | 1 | 1 | ||
| TWT615 | - | hypothetical protein | 1 | 1 | ||
| TWT617 | - | hypothetical protein | 1 | 1 | ||
| TWT618 | wblE | Putative transcription reg. protein | 1 | 1 | ||
| TWT619 | - | hypothetical protein | 1 | 1 | ||
| TWT621 | - | hypothetical protein | 1 | 1 | ||
| TWT624 | - | WND-containing WiSP family protein | 1 | 1 | ||
| TWT653 | - | membrane protein | 2 | 1 | 1 | |
| TWT673 | - | hypothetical protein | 1 | 1 | ||
| TWT679 | - | hypothetical protein | 3 | 3 | ||
| TWT704 | - | hypothetical protein | 2 | 2 | ||
| TWT722 | - | hypothetical protein | 1 | 1 | ||
| TWT751 | - | hypothetical protein | 1 | 1 | ||
| TWT762 | - | hypothetical protein | 2 | 2 | ||
| TWT773 | - | hypothetical protein | 1 | 1 | ||
| TOTAL | 72 (100%) | 27 (37.50%) | 43 (59.70%) | 2 (2.80%) | ||
The values correspond to the number of strains for which mentioned parameters were found different to that observed in T. whipplei Twist. (nt): nucleotide.