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. Author manuscript; available in PMC: 2008 Nov 10.
Published in final edited form as: Virology. 2007 Jul 16;368(1):73–82. doi: 10.1016/j.virol.2007.06.017

Figure 1.

Figure 1

Generation of V mutant rRSV. (a) Genomic structure of V mutant rRSV. (b) Expression of viral proteins in rRSV-infected cells. Vero cells were infected by the indicated viruses at a MOI of 3. Cell lysates were harvested at 16 h p.i. and separated by SDS-PAGE. Viral proteins were detected by Western blot analysis using antibodies directed against RSV structural proteins (top), V (middle), or NS1 and NS2 (bottom). (c) Electropherograms of sequences of V editing sites. Total RNA was extracted from cells infected by PIV5, ΔNS1/2-Vwt, and ΔNS1/2-Vmut at a MOI of 3 for 16 hours and subjected to RT-PCR using the V cloning primers. PCR fragments were isolated and sequenced.