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Viral RNA production in rRSV-infected cells. HEp-2 cells were infected by rA2 (a), ΔNS1/2 (b), ΔNS1/2-Vwt (c), or ΔNS1/2-Vmut (d) at a MOI of 3. Total cellular RNA was harvested at 4 hour intervals as indicated and subjected to Northern blot analysis using a radiolabeled DNA probe to RSV N. Viral RNA species are indicated on the right. G/AG, genome/antigenome.
