. Author manuscript; available in PMC: 2007 Nov 15.

Published in final edited form as: Cytogenet Genome Res. 2004;105(2-4):285–291. doi: 10.1159/000078200

Table 3.

DNA methylation status in cloned mammals

Reference	Species	Donor cells	DNA sequence	Degree of DNA methylation^a	Detection method
Kang et al., 2001b	Pig	Fetal fibroblasts	Centromeric satellite	Embryos: C=A	Bisulfite
			PRE-1 (euchromatic repeat)	Embryos C<A
Dean et al., 2001	Bovine	Fetal fibroblasts	Whole genome	Embryos: C>A	Immunofluorescence^b
Bourc'his et al., 2001	Bovine	Adult fibroblasts	Whole genome	Embryos: C>A	Immunofluorescence^b
Kang et al., 2001a	Bovine	Fetal fibroblasts	Satellite I, Satellite II, SINE and 18S rRNA	In all sequences, Embryos D=C>A	Bisulfite
Kang et al., 2002	Bovine	Fetal fibroblasts	Tissue specific promoters	Embryos: C=A	Bisulfite
Cezar et al., 2003	Bovine	Genital ridge cells	Whole genome	Summary of 4 donor cell types	Reverse phase HPLC, restriction enzyme
		Fetal skin cells		Aborted fetuses: C<<A
		Adult skin cells		Live fetuses: C<A
		Fetal and adult cumulus cells		Adults: C=A

A: age-matched controls derived from fertilization, C: cloned animals and D: the donor animals.

Immunofluorescence with anti-5-methylcytosine antibody.