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. Author manuscript; available in PMC: 2008 Oct 15.
Published in final edited form as: Dev Biol. 2007 Aug 15;310(2):317–328. doi: 10.1016/j.ydbio.2007.07.042

Figure 3. Sensory neurons derived from epibranchial placodes innervate taste buds.

Figure 3

(A) Confocal z-stack of the interior of the upper jaw of an epibranchial placode GFP-graft recipient at stage 41. GFP-positive rami of the VIIth nerve (arrows) branch to contact taste buds labeled with anti-calretinin (red). Anterior is to the top. (B-D) Transverse sections through calretinin-positive taste buds reveal that GFP-labeled fibers (green) form a large nerve plexus beneath each taste bud and extend fine processes (arrows) apically into the buds. (E) GFP-positive fibers (arrows) within taste buds (outlined by white dashed lines) are also positive for anti-acetylated tubulin (AT, red) in (F). (G) Merged image shows that GFP label co-localizes with AT. Nuclei are stained blue with Hoechst. Anti-GFP = green. Scale bar in A = 100μm; B-G = 25 μm.