Figure 1.

Expansion of LTC-IC (•) and CFC (○) numbers (relative to input) in 10-day 100-μl serum-free cultures initiated with 200 CD34⁺ CD38⁻ cells. Input LTC-IC and CFC numbers were 8.2 ± 3.3 and 7.3 ± 2.6 per 100 CD34⁺ CD38⁻ cells, respectively. A relative cytokine dose of 1 represents SF at 10 ng/ml, FL at 10 ng/ml, IL-3 at 2 ng/ml, IL-6 at 2 ng/ml, and G-CSF at 2 ng/ml. Points represent the mean ± SEM of data from three to seven experiments performed with three to five bone marrow samples.