Skip to main content
. 1997 Apr 29;94(9):4698–4703. doi: 10.1073/pnas.94.9.4698

Figure 2.

Figure 2

Size distribution (bars) and cumulative frequencies (symbols) of clones generated from a representative experiment in which single PI− AnnexinV− CD34+ CD38− cells were cultured for 10 days in serum-free medium supplemented with (i) FL at 300 ng/ml, SF at 300 ng/ml, IL-3 at 60 ng/ml, IL-6 at 60 ng/ml, and G-CSF at 60 ng/ml (solid bars and ▪, n = 59 wells) or (ii) FL at 30 ng/ml, SF at 30 ng/ml, IL-3 at 6 ng/ml, IL-6 at 6 ng/ml, and G-CSF at 6 ng/ml (open bars and □, n = 59 wells) (A) or (iii) FL at 300 ng/ml, SF at 300 ng/ml, and IL-3 at 60 ng/ml (solid bars, •, n = 59 wells) or (iv) FL at 10 ng/ml, SF at 10 ng/ml, and IL-3 at 60 ng/ml (open bars and ○, n = 57 wells) (B). At the end of the 10 days, all cells from all clones generated under a given set of conditions were pooled and assayed for their content of CFCs and LTC-ICs. The results of these assays confirmed the results shown in Fig. 1 (for the comparison undertaken in A) and in Table 1 (for the comparison undertaken in B) (see Results).