Number of colonies generated from 200 CD34+ CD38− (A), CD34+ CD45RA+ CD71− (B), or CD34+ CD45RA− CD71+ cells (C) plated directly in methylcellulose containing different concentrations of the same mixture of five cytokines used in the experiments shown in Fig. 1. Points for erythroid (•, from CFU-E and BFU-E), mixed (○, from CFU-GEMM), and myeloid (▴, from CFU-GM) colonies represent the mean ± SEM of values obtained from four experiments.