Table 1.
Effect of antibodies to recombinant FA-1 antigen on sperm–zona pellucida binding in mice
| Treatment | Amount, μg/120 μl | Number of ova tested* | Sperm bound per egg |
|---|---|---|---|
| Antibody to | 5 | 52 | 0‡ |
| recombinant FA-1 | 10 | 105 | 0‡ |
| Anti-FA-1 mAb | 10 | 48 | 2 ± 0.2 |
| Preimmune | 5 | 38 | 40 ± 6 |
| immunoglobulins | 10 | 41 | 39 ± 7 |
| Control rabbit | 5 | 51 | 43 ± 6 |
| immunoglobulins† | 10 | 62 | 38 ± 9 |
| Myeloma control | |||
| immunoglobulins | 10 | 53 | 41 ± 7 |
| Untreated control | — | 102 | 42 ± 8 |
*
Pooled from 3–5 different experiments performed on various days using sperm and oocytes from different groups of mice.
†
Control rabbit immunoglobulins were collected from rabbits immunized with Freund’s adjuvant alone.
‡
Versus untreated/preimmune/control rabbit immunoglobulins/myeloma control immunoglobulins, P < 0.0001; others were nonsignificant, P > 0.05.