Figure 1.

Schematic diagram of the plasmids α-SCSB-CAT, α-SCSB-NEO and α-SCSB-LUC used to transform T. vaginalis. Sequences of the 5′ and 3′ ends of the α-SCSB UTRs are represented by a heavy line. → Represents the start of the α-SCSB coding region, which is followed by the first 82 bp of the α-SCSB gene(▪) fused in-frame to the reporter gene (▨). The coding regions of cat, neo, and luc are flanked by KpnI/BamHI sites that were used to prepare the constructs using the pBluescript (pBS) vector. Relevant restriction enzyme sites are shown.