Figure 2.

CAT expression in T. vaginalis cells transformed by electroporation at 350 volts and 960 μFd. TLC demonstrating unacetylated ¹⁴-C chloramphenicol (Cm) and monoacetylated forms of the substrate (M) derived following incubation of T. vaginalis extracts with ¹⁴-C chloramphenicol and acetyl CoA. The following T. vaginalis extracts were used in CAT assays: Lane 1, nonelectroporated extracts containing 5 × 10 units of commercially available CAT (Pharmacia) as a positive control; Lane 2, nonelectroporated extracts without addition of commercially available CAT; Lane 3, extracts electroporated in the absence of DNA; Lane 4, extracts electroporated in the presence of the α-SCSB-CAT construct; Lane 5, extracts electroporated in the presence of the plasmid pBLCAT.