Figure 5.

Southern blot analysis of total T. vaginalis DNA from clone 1, transformed with the αSCSB-NEO construct and selected using 50 μg/ml paromomycin. DNA (1 μg) was digested with a single restriction enzyme, as indicated (lanes 1–4) or a combination of two restriction enzymes, as indicated (lanes 5–7), and hybridized with a ³²P-labeled neo gene probe. See Fig. 1 for restriction enzyme map of the construct.