Figure 3.

Electron micrographs or photomicrographs (Insets) of longitudinal sections of intact MGAs in physiological saline (A) or severed in physiological saline (B) or severed in Ca²⁺-free saline with 1 mM EGTA (C) and fixed 60 min later. AP, MGA axoplasm; MT, microtubules; M, mitochondria; AL, axolemma; GP, adaxonal layer of gliaplasm; GN, glial nucleus; (boxed area of Inset in A, glial layers; boxed area of inset in B, small pore at cut end; V, remnants of larger vesicles (≥2 μm) often observed to burst when MGAs severed in physiological saline were exposed to fixative and imaged with DIC. (Bar = 1 μm for A and C; 0.5 μm for B; and 20 μm for Insets A–C.)