Figure 3.

Hippocampal morphology in fyn-deficient and fyn-rescue mice. (A) Nissl staining of rostral and caudal coronal hippocampal sections of wild-type (wt), fyn-deficient (fyn), and fyn-rescue (rescue) mice. Characteristic abnormal arrangements (arrowheads) of rostral dentate gyrus (DG) and caudal CA3 region in fyn-deficient mice were also observed in the rescue mice. (Bars = 500 μm.) (B) Dendritic organization of the CA1 field in rescue mice. Apical dendrites of the pyramidal cells were revealed by immunohistochemical staining using anti-MAP2 antibody. CA1 pyramidal cell layer (pc) was less tightly packed and ectopic pyramidal cells (arrowheads) were frequently observed in fyn-deficient (fyn) and fyn-rescue (rescue) mice. Dendrites ran across the stratum radiatum (sr) and lacunosum-moleculare (lm), but their lengths were shorter than in wild-type litter mates. (Bar = 50 μm.)