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. Author manuscript; available in PMC: 2008 Sep 1.
Published in final edited form as: J Inorg Biochem. 2007 Jun 13;101(9):1265–1269. doi: 10.1016/j.jinorgbio.2007.06.004

Fig 1. Generation of ROS by metal sulfates in HN cell culture.

Fig 1

The evolution of reactive oxygen species (ROS) using 2′,7′-dichlorofluorescein diacetate (H2DCFDA) assay was quantified in 2 week old HN cells 3.5 days after initial treatment with 100 nM magnesium-, iron-, aluminum-, magnesium plus aluminum- or iron- plus aluminum-sulfate [1]. These were compared to age-matched untreated controls containing no metal sulfate (value arbitrarily set to 1.0; dashed horizontal line) [1]. Because aluminum-sulfate alone and iron- plus aluminum-sulfate together were found to generate the most ROS using the H2DCFDA assay (Fig.1), these metal sulfates were next used to study their effects on miRNA expression in HN cells. Data are expressed as relative fluorescence yield, defined as fold-changes over 1.0 (dashed horizontal line). N=5; *p<0.05; **p<0.01 (ANOVA).