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MIF is highly expressed in pancreatic β cell lines. Northern blotting analyses of MIF mRNA (0.6 kb) and β-actin mRNA (1.8 kb) expression in two insulin-producing cell lines (INS-1 and βTC3), an insulin and somatostatin cell line (RIN1027-B2), a glucagon-producing cell line (InR1-G9), and nonpancreatic cell lines (JEG-3 and COS-1). Total RNA (10 μg) was prepared and analyzed as described in Methods. The MIF transcript is detected in high abundance in the insulin-producing cell lines.
