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. 1997 Apr 29;94(9):4788–4793. doi: 10.1073/pnas.94.9.4788

Figure 4.

Figure 4

(a) Intestinal lamina propria macrophages isolated from toxin A-injected loops show enhanced release of TNFα. Ileal loops were injected with either toxin A or buffer. After 1 hr, LPMs were purified and cultured and TNFα release was determined as described. Each data point = mean ± SEM of values derived from six to eight rats, each with quadruplicate determinations for each experimental condition. ∗, P < 0.05; ∗∗, P < 0.01 vs. control. (b) Pretreatment of rats with the SP antagonist CP-96,345 reduces toxin A-induced TNFα release from LPMs. Rats were injected i.p. with either saline or saline containing CP-96,345 (2.5 mg/kg) or CP-96,344 (2.5 mg/kg) 10 min before administration of toxin A or buffer into ileal loops. After 1 hr LPMs were isolated and cultured and TNFα release after 4 hr was determined as described. Bars = mean ± SEM of values derived from six to eight rats, each with quadruplicate determinations for each experimental condition. ∗, P < 0.05; ∗∗, P < 0.01 vs. control; +, P < 0.05 vs. toxin A.