Figure 1.

Sequence and phylogenetic analysis. (A) Deduced amino acid sequence of tomato Cel3 aligned with tomato Cel1 and Cel2 mature proteins. Amino acid identity is indicated by asterisks and conserved changes by dots. Potential N-glycosylation sequences in Cel3 are overlined. (B) Schematic representation of tomato Cel3 protein structure. Cel3 consists of a charged N terminus (shaded box), a transmembrane domain (solid box), and the catalytic core (hatched box). (C) Hydropathic profile of Cel3 deduced protein. (D) Phylogenetic comparison of plant EGase deduced proteins after removal of signal sequences. Sequences and GenBank accession numbers: avocado, Cel1 (M17634M17634); bean, BAC1 (M57400M57400); elder, JET1 (X74290X74290); pea, EGL1 (L41046L41046); peach, Cel1 (X96853X96853); pepper, Cel1 (X87323X87323), Cel2 (X97190X97190), and Cel3 (X97189X97189); poplar, Cel1 (D32166D32166); tomato, Cel1 (U13054U13054), Cel2 (U13055U13055), and Cel4 (also called TPP18; U20590U20590).