Figure 2.

DNA and RNA gel blot analysis. (A) DNA gel blot of 10-μg aliquots of genomic DNA digested with the indicated restriction enzyme. The gel blot was hybridized with a ³²P-labeled 1117-nucleotide Cel3 cDNA fragment in 50% formamide, 0.9 M NaCl (6× SSPE) at 36°C, with final wash in 0.075 M NaCl (0.5× SSC) at 53°C (27°C below T_m). (B) RNA gel blot of 3 μg of stem poly(A)⁺ RNA hybridized with an antisense Cel3 riboprobe in 50% formamide, 0.75 M NaCl (5× SSPE) at 59°C, with final wash in 0.015 M NaCl (0.1× SSC) at 49°C (23°C below T_m).