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. 1997 Apr 29;94(9):4794–4799. doi: 10.1073/pnas.94.9.4794

Figure 3.

Figure 3

Western blots of uninduced and induced E. coli proteins (100 ng per lane) and tomato proteins (50 μg per lane) probed with Cel3 antibody (1:7,500) affinity-purified against recombinant Cel3 protein blotted to transfer membrane (A) or against transfer membrane alone blocked with BSA (B). Lanes: 1, uninduced E. coli; 2, E. coli induced with isopropyl β-d-thiogalactoside; 3, tomato soluble proteins; 4, tomato microsomal membranes; 5, tomato cell wall proteins; 6–9, microsomal membranes treated with no trypsin, no Triton (lane 6), no trypsin, 0.1% Triton (lane 7); 500 units of trypsin, no Triton (lane 8); 500 units of trypsin, 0.1% Triton (lane 9).