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Distribution of membrane marker enzymes in a sucrose density gradient used to separate tomato root microsomal membranes. (A) Total protein determined using Bradford assay and sucrose concentration measured by refractometry. (B) NADH:cytochrome c reductase (ER) and vanadate-sensitive ATPase (plasma membrane). (C) Bafilomycin-sensitive ATPase (tonoplast) and latent UDPase (Golgi). Marker enzyme and protein assays performed as described (13).
