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Western blots of tomato root microsomal membrane density gradient fractions probed with antibodies to plasma membrane ATPase (100 kDa), ER-localized BiP (70 kDa), and 58-kDa subunit of tonoplast ATPase (58 kDa) (A); or affinity-purified Cel3 antibody (B). (C) CM-cellulase activity determined using viscometry in the absence (striped bars) or presence (solid bars) of 0.1% Triton X-100. (D) Triton-activated CM-cellulase activity calculated from C.
