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. Author manuscript; available in PMC: 2008 Sep 1.
Published in final edited form as: Matrix Biol. 2007 May 31;26(7):541–553. doi: 10.1016/j.matbio.2007.05.006

Figure 3.

Figure 3

Figure 3

Figure 3

Elevated endogenous BMP activity in DZC. (A) Expression levels of Noggin, a BMP antagonist were compared in SZC and DZC immediately after harvest (day 0) or on day 7 of culture by qRT-PCR as described in Experimental Procedures. (B) Presence of phosphorylated (active) Smad 1/5/8 and (C) total Smad were compared in SZC and DZC that were treated with no growth factor (naïve) or with recombinant BMP2 (200 ng/ml) or recombinant Noggin (200 ng/ml) for 4 hr by Western blotting as described in Experimental Procedures. The pSmad1/5/8 and total Smad1 ran at 60kDa, while GAPDH (used as a loading control) ran at 38kDa.